The continuous development of maritime industry has brought a marked increase in the construction land of port areas. The ecological problems of ports in China have received more and more attention. Frequent human activities had caused dramatic changes of land use pattern in port areas in the past decades, which brought a significant impact on local environment. Together with the RS, GIS technologies, we relied on land use pattern analysis and ecosystem service value evaluation methods to analyze the ecosystem service value changes of study area. The authors found that from 1995 to 2015, the area of ecological lands reduced significantly in the study area. The total ecosystem service value of the study area was 3.84×107 Yuan in 1995 and 2.25×107 Yuan in 2015, with a reduction of 1.59×107 Yuan from 1995 to 2015. This study aims to provide references for the ecological environment protection in port areas.