Simulation model is usually used for the process modelling and analysis of the systems where other mathematical techniques are hardly to use. Discrete event simulation could be applied at those type of processes where the change in the system does not occur continuously but only when a significant event happens. It is typical for various queuing systems analysis. This contribution deals with the application of simulation program SIMUL8 to the analysis of the cash-desk utilization in a selected shop of the retail chain. The main aim is to model the system and to use the simulation model to set the optimal number of cashdesks and test several “what-if” situations. The advantage of SIMUL8 consists of its simplicity and interpretability of achieved results. Our results showed that in case of bigger purchase more than 16 cash desks are necessary to open to achieve less than 10 minutes customers’ waiting times.