In most classic vehicle routing problems, the main goal is to minimise the total travel time or distance while, the green vehicle routing problem, in addition to the stated objectives, also focuses on minimising fuel costs and greenhouse gas emissions, including carbon dioxide emissions. In this research, a new approach in Pollution Routing Problem (PRP) is proposed to minimise the CO2 emission by investigating vehicle weight fill level in length of each route. The PRP with a homogeneous fleet of vehicles, time windows, considering the possibility of split delivery and constraint of minimum shipment weight that must be on the vehicle in each route is investigated simultaneously. The mathematical model is developed and implemented using a simulated annealing algorithm which is programmed in MATLAB software. The generated results from all experimentsdemonstrated that the application of the proposed mathematical model led to the reduction in CO2 emission.