The tank sloshing is simulated at different liquid levels and different wave excitation. At the same time, the motion responses of one point mooring ship considering sloshing loads are investigated in frequency domain. Based on Volume of Fluid (VOF) method, the numerical simulation of tank sloshing is discussed by the FLUENT software. The sloshing forces and moments on the cabin’s walls are obtained by the sloshing pressure at different liquid levels and different time nodes. Then, the motion equation of one point mooring ship considering the sloshing loads is established in frequency domain. The sloshing forces and moments are equivalent to the hydrodynamic coefficients of the hull. And the additional mass, damping coefficients and stiffness coefficient of the original hull are obtained by AQWA software. The ship motion responses equation is solved by Runge Kutta method. The contrasting results of ship motion and mooring strength with different tank filling levels indicate that the maximum amplitude of the ship motion response deviates and the frequency corresponding to the amplitude changes accordingly.